Yakko, Wakko, and Dot are the mischievous main characters of which animated sketch comedy series, known for its slapstick humor, pop culture parodies, and educational segments?

The animated sketch comedy series featuring the mischievous trio of Yakko, Wakko, and Dot is indeed Animaniacs (Review). These three zany siblings, often referred to as the Warner Brothers and the Warner Sister, are the central protagonists of the show. They are depicted as classic 1930s cartoon characters who were locked away in the Warner Bros. water tower for decades due to their chaotic and unpredictable nature, only to escape and wreak havoc across the studio lot and beyond.

Animaniacs, created by Tom Ruegger and executive produced by Steven Spielberg, is renowned for its unique blend of rapid-fire slapstick humor, clever wordplay, and extensive pop culture parodies that appeal to both children and adults. The show frequently broke the fourth wall and satirized various aspects of media, politics, and celebrity culture. Beyond the comedic segments, Animaniacs also incorporated educational elements, often through catchy musical numbers. Songs like "Yakko's World" famously taught geography, while other segments covered history, science, and even existential concepts in an accessible and entertaining way, adhering to requirements for children's programming while maintaining its irreverent spirit.

This variety show format, featuring a large cast of characters in addition to the Warners, allowed for diverse storytelling and comedic styles across its original run from 1993 to 1998, and its later revival. The show's ability to deliver smart humor alongside valuable lessons cemented its place as a beloved and influential animated series.